Iyo iri kubuda autologous cell therapy uchishandisa platelet-rich plasma (PRP) inogona kuita basa rekubatsira muzvirongwa zvakasiyana-siyana zvekurapa mushonga.Pane kudiwa kwepasi rose kwemaitiro ekugadzirisa matishu ekurapa varwere vane musculoskeletal (MSK) uye zvirwere zvemuzongoza, osteoarthritis (OA) uye maronda asingaperi akaoma uye anomira.PRP therapy inobva pakuti platelet growth factor (PGF) inotsigira maronda ekupora uye kugadzirisa cascade (kuzvimba, kuwedzera uye kugadzirisa).Nhamba dzakasiyana dzakasiyana dzePRP dzakaongororwa kubva kuvanhu, in vitro uye zvidzidzo zvemhuka.Nekudaro, kurudziro ye in vitro uye zvidzidzo zvemhuka zvinowanzo tungamira kune akasiyana mhedzisiro yekiriniki, nekuti zvakaoma kushandura isiri-kiriniki yekutsvagisa mhinduro uye nzira yekurudziro mukurapa kwekiriniki yevanhu.Mumakore achangopfuura, kufambira mberi kwakaitwa mukunzwisisa pfungwa yePRP tekinoroji uye biological agents, uye mitsva yekutsvagisa mirairo uye zviratidzo zvitsva zvakarongwa.Muchidzidzo ichi, tichakurukura kufambira mberi kwekupedzisira mukugadzirira uye kuumbwa kwePRP, kusanganisira platelet dose, leukocyte basa uye innate uye adaptive immune regulation, 5-hydroxytryptamine (5-HT) mhedzisiro uye marwadzo.Pamusoro pezvo, takakurukura nezvePRP nzira ine chekuita nekuzvimba uye angiogenesis panguva yekugadziriswa kwematishu uye kuberekwa patsva.Pakupedzisira, tichaongorora migumisiro yeimwe mishonga pane PRP basa.
Autologous platelet-rich plasma (PRP) chikamu chemvura che autologous peripheral ropa mushure mekurapwa, uye iyo platelet concentration yakakwira kupfuura yekutanga.PRP therapy yakashandiswa kune zviratidzo zvakasiyana-siyana kwemakore anopfuura 30, zvichiita kuti kufarira kukuru kune zvingaita zve autogenous PRP mumishonga yekuvandudza.Izwi rekuti orthopedic biological agent rachangobva kuunzwa kurapa musculoskeletal (MSK) zvirwere, uye rakawana mhedzisiro inovimbisa mukukwanisa kuberekwa patsva kweheterogeneous bioactive PRP cell musanganiswa.Parizvino, kurapwa kwePRP inzira yakakodzera yekurapa ine mabhenefiti ekiriniki, uye mhedzisiro yemurwere yakashumwa inokurudzira.Zvisinei, kusawirirana kwemigumisiro yevarwere uye ruzivo rutsva rwakaisa matambudziko kune practicability yekliniki yekushandiswa kwePRP.Chimwe chezvikonzero chinogona kunge chiri nhamba uye kusiyanisa kwePRP uye PRP-mhando masisitimu pamusika.Midziyo iyi yakasiyana maererano nePRP yekuunganidza vhoriyamu uye chirongwa chekugadzirira, zvichikonzera akasiyana ePRP maitiro uye biological agents.Mukuwedzera, kushayikwa kwekubvumirana pamusoro pekumisikidzwa kwePRP gadziriro yegadziriro uye mushumo wakazara wevashandi vehupenyu mukushandiswa kwekliniki zvakakonzera kusawirirana kwemigumisiro yemushumo.Kuedza kwakawanda kwakaitwa kuratidza uye kuisa PRP kana zvigadzirwa zvakatorwa neropa mumashandisirwo emishonga yekuvandudza.Pamusoro pezvo, zvinobuda muplatelet, zvakaita semaplatelet lysates, zvakakurudzirwa kutsvagisa orthopedic uye in vitro stem cell.
Imwe yemashoko ekutanga pamusoro pePRP yakabudiswa mu 2006. Chinangwa chikuru chekuongorora uku ibasa uye maitiro ekuita kweplatelet, mhedzisiro yePRP pane imwe neimwe nhanho yekuporesa kupora, uye basa guru replatelet-derived growth factor. mune zvakasiyana-siyana PRP zviratidzo.Muchikamu chekutanga chePRP tsvakurudzo, chinonyanya kufarira muPRP kana PRP-gel kwaiva kuvapo uye mabasa chaiwo ezvizhinji zveplatelet kukura zvinhu (PGF).
Mupepa rino, tichakurukura zvakanyanya nezvekuvandudzwa kweakasiyana PRP particle zvimiro uye platelet cell membrane receptors uye mhedzisiro yavo pane innate uye inogadzirisa immune system immune regulation.Mukuwedzera, basa remasero ega ega angave aripo muPRP yekurapa vial uye simba ravo pakugadzirisa mutsara wetishu richakurukurwa zvakadzama.Mukuwedzera, kufambira mberi kwekupedzisira mukunzwisisa PRP biological agents, platelet dose, zvakananga zvinokonzerwa nemasero machena eropa, uye migumisiro yePGF concentration uye cytokines pamigumisiro yehutano ye mesenchymal stem cells (MSCs) ichatsanangurwa, kusanganisira PRP inotarisa zvakasiyana. sero uye matishu nharaunda mushure mesero chiratidzo chekufambisa uye paracrine mhedzisiro.Saizvozvo, isu tichakurukura iyo PRP nzira ine chekuita nekuzvimba uye angiogenesis panguva yekugadziriswa kwematishu uye kuberekwa patsva.Pakupedzisira, tichaongorora maitiro eanalgesic ePRP, mhedzisiro yemamwe madhiragi pane basa rePRP, uye kusanganiswa kwePRP uye zvirongwa zvekuvandudza.
Nheyo dzekutanga dzekiriniki yeplatelet-rich plasma therapy
Kugadzirira kwePRP kuri kuwedzera kuve kwakakurumbira uye kushandiswa zvakanyanya mune dzakasiyana siyana dzekurapa.Nheyo yekutanga yesainzi yePRP kurapwa ndeyokuti jekiseni remaplatelet akaiswa panzvimbo yakakuvara inogona kutanga kugadziriswa kwetishu, kuumbwa kwezvitsva zvinobatanidza uye kuvakwazve kwekutenderera kweropa nekuburitsa zvinhu zvakawanda zvehupenyu (kukura, cytokines, lysosomes) uye adhesion mapuroteni ane basa rekutanga hemostatic cascade reaction.Mukuwedzera, mapuroteni eplasma (eg fibrinogen, prothrombin, uye fibronectin) aripo muzvikamu zveplatelet-poor plasma (PPPs).PRP concentrate inogona kukurudzira kusunungurwa kwe hyperphysiological yekukura zvinhu kuti utange kupora kwekukuvara kusingaperi uye kukurumidza kugadziriswa kwekukuvara kwakanyanya.Pamatanho ese ekugadzirisa matishu maitiro, akasiyana ekukura zvinhu, ma cytokines uye emunharaunda zviito zvinodzora zvinosimudzira akawanda ekutanga maseru mabasa kuburikidza ne endocrine, paracrine, autocrine uye endocrine nzira.Kubatsira kukuru kwePRP kunosanganisira kuchengetedzwa kwayo uye inyanzvi yekugadzirira tekinoroji yezvino zvekutengesa zvigadzirwa, izvo zvinogona kushandiswa kugadzira biological agents zvinogona kushandiswa zvakanyanya.Zvinonyanya kukosha, zvichienzaniswa neyakajairwa corticosteroids, PRP ndeye autogenous chigadzirwa chisina inozivikanwa mhedzisiro.Zvisinei, hapana mutemo wakajeka pamusoro pekugadzirisa uye kuumbwa kweiyo injectable PRP kuumbwa, uye kuumbwa kwePRP kune kuchinja kukuru mumaplatelet, white blood cell (WBC) content, red blood cell (RBC) kusvibiswa, uye PGF concentration.
PRP terminology uye kupatsanura
Kwemakumi emakore, kusimudzirwa kwezvigadzirwa zvePRP zvakashandiswa kukurudzira kugadziriswa kwetishu uye kuvandudzwa kwave kwakakosha kutsvagisa nzvimbo yebiomaterials uye sainzi yezvinodhaka.Iyo matishu inoporesa cascade inosanganisira vazhinji vatori vechikamu, kusanganisira maplatelet nekukura kwavo zvinhu uye cytokine granules, masero machena eropa, fibrin matrix uye mamwe akawanda synergistic cytokines.Mune iyi cascade process, yakaoma coagulation process ichaitika, kusanganisira platelet activation uye inotevera densification uye α- Kuburitswa kwezviri mukati meplatelet particles, aggregation yefibrinogen (yakaburitswa nemaplatelet kana mahara muplasma) mufibrin network, uye kuumbwa. chirwere cheplatelet embolism.
"Universal" PRP inofananidzira kutanga kwekuporesa
Pakutanga, shoko rokuti “platelet-rich plasma (PRP)” rainzi platelet concentrate rinoshandiswa mukurapa kwokuwedzera ropa, uye richiri kushandiswa nanhasi.Pakutanga, izvi zvigadzirwa zvePRP zvaingoshandiswa sefibrin tissue adhesive, nepo maplatelet aingoshandiswa kutsigira yakasimba fibrin polymerization kunatsiridza kuiswa chisimbiso kwetishu, kwete sechinhu chinoporesa.Mushure meizvozvo, tekinoroji yePRP yakagadzirirwa kutevedzera kutangwa kweanopodza cascade.Zvadaro, tekinoroji yePRP yakapfupikiswa kuburikidza nekugona kwayo kuunza uye kuburitsa zvinhu zvekukura mukati meiyo microenvironment.Kufarira uku kwePGF kuendesa kunowanzovanza basa rakakosha rezvimwe zvikamu mune izvi zvinobva muropa.Kushingairira uku kunowedzerwa nekuda kwekushaikwa kwedata resainzi, zvitendero zvisinganzwisisike, zvishuwo zvekutengesa uye kushaikwa kwekumisikidza uye kupatsanura.
Iyo biology yePRP concentrate yakaoma seropa pachayo, uye inogona kunge yakanyanya kuoma kupfuura zvinodhaka zvechivanhu.PRP zvigadzirwa zvinorarama biomaterials.Migumisiro yekliniki yePRP yekushanda inobva pane zvemukati, zvepasi rose uye zvinogadziriswa zveropa remurwere, kusanganisira zvimwe zvakasiyana-siyana zvemasero zvinogona kunge zviripo mumuenzaniso wePRP uye microenvironment yemunharaunda ye receptor, iyo inogona kunge iri mumamiriro akaoma kana asingagumi.
Pfupiso yekuvhiringidza PRP terminology uye yakarongwa classification system
Kwemakore akawanda, vashandi, masayendisiti nemakambani vave vachitambudzwa nekusanzwisisana kwekutanga uye kukanganisa kwezvigadzirwa zvePRP nemashoko avo akasiyana.Vamwe vanyori vakatsanangura PRP seplatelet-chete, asi vamwe vakaratidza kuti PRP inewo masero matsvuku eropa, masero machena akasiyana-siyana eropa, fibrin uye bioactive mapuroteni ane kuwedzera kwehuwandu.Naizvozvo, akawanda akasiyana PRP biological agents akaunzwa mukiriniki maitiro.Zvinoodza moyo kuti mabhuku anowanzo shaya tsananguro ine udzame yezvinoita biological agents.Iko kutadza kwekugadzirwa kwechigadzirwa kumisa uye kunotevera kurongedza sisitimu yekusimudzira kwakatungamira mukushandiswa kwenhamba yakawanda yezvigadzirwa zvePRP zvinotsanangurwa nemazwi akasiyana uye zvipfupiso.Hazvishamisi kuti kuchinja mukugadzirira kwePRP kunotungamirira kumigumisiro yevarwere isingaenderani.
Kingsley akatanga kushandisa izwi rokuti "platelet-rich plasma" muna 1954. Makore akawanda gare gare, Ehrenfest et al.Yekutanga classification system yakavakirwa pamatatu makuru akasiyana (platelet, leukocyte uye fibrin content) yakarongwa, uye akawanda PRP zvigadzirwa zvakakamurwa muzvikamu zvina: P-PRP, LR-PRP, pure platelet-rich fibrin (P-PRF) uye leucocyte. PRF yakapfuma (L-PRF).Izvi zvigadzirwa zvinogadzirirwa neatomatiki yakavharwa system kana manual protocol.Zvichakadaro, Everts et al.Kukosha kwekutaura masero machena eropa mukugadzirira kwePRP kwakasimbiswa.Vanokurudzirawo kushandiswa kwemazwi akakodzera kuratidza kusashanda kana kushandiswa kweshanduro dzePRP gadziriro uye platelet gel.
Delong et al.yakaronga PRP classification system inonzi maplatelet, activated white blood cells (PAW) zvichienderana nehuwandu hwehuwandu hwemaplatelet, kusanganisira mana ekugadzirisa maplatelet.Mamwe maparamendi anosanganisira kushandiswa kwemaplatelet activator uye kuvapo kana kusavapo kwemasero machena eropa (kureva neutrophils).Mishra et al.Iyo yakafanana classification system inokurudzirwa.Makore mashoma gare gare, Mautner nevamwe vake vakatsanangura yakawedzera kurongeka uye yakadzama classification system (PLRA).Munyori akaratidza kuti zvakakosha kutsanangura mhedziso yeplatelet count, white blood cell content (yakanaka kana yakaipa), neutrophil percentage, RBC (yakanaka kana yakaipa) uye kana exogenous activation inoshandiswa.Muna 2016, Magalon et al.Iyo DEPA kupatsanurwa kwakavakirwa pamuyero wejekiseni replatelet, kugona kwekugadzira, kuchena kwePRP kwakawanikwa uye activation process yakaburitswa.Mushure mezvo, Lana nevamwe vake vakaunza MARSPILL classification system, vachitarisa pamaperipheral blood mononuclear cells.Munguva pfupi yapfuura, Sainzi Kumisikidzwa kweKomiti yakakurudzira kushandiswa kweiyo Classification system yeInternational Society for Thrombosis uye Hemostasis, iyo yakavakirwa panhevedzano yezvibvumirano zvekumisikidza kushandiswa kwezvigadzirwa zveplatelet mumashandisirwo emishonga yekuvandudza, zvinosanganisira chando uye chakanyungudutswa cheplatelet zvigadzirwa.
Zvichienderana nePRP classification system inokurudzirwa nevashandi vakasiyana-siyana nevatsvakurudzi, kuedza kwakawanda kusina kubudirira kuenzanisa kugadzirwa, tsanangudzo uye maitiro ePRP kuti ashandiswe nevarapi vanogona kutora mhedziso yakarurama, iyo ingangodaro isingaitiki mumakore mashomanana anotevera Mukuwedzera. , tekinoroji yezvigadzirwa zvePRP zvekiriniki zvinoramba zvichikura, uye data yesainzi inoratidza kuti gadziriro dzakasiyana dzePRP dzinodiwa kurapa zvirwere zvakasiyana-siyana pasi pemamiriro chaiwo.Naizvozvo, isu tinotarisira kuti ma paramita uye akasiyana eakanaka PRP kugadzirwa acharamba achikura mune ramangwana.
PRP yekugadzirira nzira iri kuenderera mberi
Zvinoenderana nePRP terminology uye tsananguro yechigadzirwa, akati wandei masisitimu anoburitswa kune akasiyana PRP mafomati.Nehurombo, hapana kubvumirana pane yakazara classification system yePRP kana chero imwe autologous ropa uye zvigadzirwa zveropa.Nenzira yakanaka, hurongwa hwekugadzirisa hunofanira kubhadhara kune zvakasiyana-siyana zvePRP maitiro, tsanangudzo uye mazita akakodzera ane chokuita nemasarudzo ekurapa evarwere vane zvirwere zvakasiyana.Parizvino, mishonga yemapfupa inoparadzanisa PRP muzvikamu zvitatu: pure platelet-rich fibrin (P-PRF), leucocyte-rich PRP (LR-PRP) uye leucocyte-deficient PRP (LP-PRP).Kunyangwe iyo yakanyanya kujeka kupfuura iyo yakajairwa PRP chigadzirwa tsananguro, LR-PRP uye LP-PRP zvikamu zviri pachena kuti hazvina chero hunhu mune chena masero eropa.Nekuda kwemaitiro ayo ekudzivirira uye ekudzivirira ekudzivirira, masero machena eropa akanganisa zvakanyanya iyo intrinsic biology yezvirwere zvisingaperi zvinyama.Nokudaro, PRP biological agents ine chaiwo masero machena eropa anogona kukurudzira zvakanyanya kuchengetedzwa kwemuviri uye kugadziriswa kwetishu uye kuberekwa patsva.Kunyanya, lymphocytes yakawanda muPRP, inogadzira insulini-yakafanana nekukura chinhu uye inotsigira kugadziriswa kwetishu.
Monocytes uye macrophages anoita basa rakakosha mukugadzirisa kwekudzivirira kwemuviri uye nzira yekugadzirisa matishu.Kukosha kweneutrophils muPRP hakuna kujeka.LP-PRP yakatemerwa seyekutanga PRP gadziriro nekuongororwa kwakarongeka kuti uwane mhedzisiro yekurapa inoshanda yemubatanidzwa OA.Zvisinei, Lana et al.Kushandiswa kweLP-PRP mukurapa kwebvi OA kunopesana, izvo zvinoratidza kuti masero machena eropa anoita basa rinokosha muhutano hwehutachiona husati hwavapo patsva, nokuti vanosunungura pro-inflammatory uye anti-inflammatory molecules.Vakaona kuti kusanganiswa kwemaneutrophils uye maplatelet akabatidzwa aive nemhedzisiro yakanaka pane yakashata pakugadzirisa matishu.Vakataurawo kuti plastiki ye monocytes yakakosha kune iyo isiri-kuzvimba uye kugadzirisa basa mukugadzirisa matishu.
Chirevo chePRP gadziriro yekugadzirira mukutsvagisa kwekiriniki haina kuenderana.Zvizhinji zvakaburitswa zvidzidzo hazvina kutsanangura nzira yekugadzirira yePRP inodiwa kuti idzokorore chirongwa.Hapana kubvumirana kwakajeka pakati pezviratidzo zvekurapa, saka zvakaoma kuenzanisa zvigadzirwa zvePRP nemigumisiro yavo yekurapa yakabatana.Muzviitiko zvakawanda zvakashumwa, kurapwa kweplatelet concentration inorongwa pasi pezwi rokuti "PRP", kunyange kune imwechete yekliniki chiratidzo.Kune dzimwe nzvimbo dzezvokurapa (zvakadai seOA uye tendinosis), kufambira mberi kwakaitwa pakunzwisisa kuchinja kwePRP gadziriro, nzira dzekuendesa, platelet function uye zvimwe zvikamu zvePRP zvinokanganisa kugadziriswa kwemavara uye kuvandudzwa kwemavara.Nekudaro, kumwe kutsvagisa kunodiwa kuti uwane kubvumirana pane PRP terminology ine chekuita nePRP biological agents kuitira kuti zvizere uye zvakachengeteka kubata mamwe ma pathologies nezvirwere.
Mamiriro ePRP classification system
Kushandiswa kweautologous PRP biotherapy kunotambudzwa nehterogeneity yePRP gadziriro, kusingawirirani kutumidza uye kusina kunaka kwehutano hwehupupuriro-based guidelines (kureva, kune nzira dzakawanda dzekugadzirira kubudisa mabhodhoro ekurapa kwekliniki).Zvinogona kufanotaurwa kuti mhedziso yePRP yemukati, kuchena uye zvipenyu hunhu hwePRP uye zvine hukama zvigadzirwa zvinosiyana zvakanyanya, uye zvinokanganisa hutano hwehupenyu uye mhedzisiro yekuyedzwa kwekiriniki.Kusarudzwa kwePRP kugadzirira mudziyo kunosuma yekutanga kiyi shanduko.Mukiriniki regenerative mushonga, varapi vanogona kushandisa maviri akasiyana PRP kugadzirira michina uye nzira.Kugadzirira kunoshandisa separator yeropa, iyo inoshanda paropa rakazara rinounganidzwa pacharo.Iyi nzira inoshandisa inoramba ichiyerera centrifuge dhiramu kana disk separation tekinoroji uye yakaoma uye yakapfava centrifuge matanho.Zvizhinji zvezvigadziri izvi zvinoshandiswa pakuvhiya.Imwe nzira ndeye kushandisa giravhiti centrifugal tekinoroji nemidziyo.High G-force centrifugation inoshandiswa kupatsanura yellow layer yeESR kubva muchikamu cheropa chine maplatelet nemasero machena eropa.Midziyo iyi yekuisa pfungwa idiki pane inoparadzanisa masero eropa uye inogona kushandiswa padivi pemubhedha.Musiyano ģ -Kumanikidza uye centrifugation nguva inotungamira kune misiyano yakakura mugoho, kutarisisa, kuchena, kugona, uye activated mamiriro emaplatelet ari ega.Mhando dzakawanda dzekutengesa PRP gadziriro yemidziyo inogona kushandiswa muchikamu chekupedzisira, zvichikonzera imwe shanduko mune zvechigadzirwa.
Kushaikwa kwekubvumirana pamusoro penzira yekugadzirira uye kusimbiswa kwePRP kunoramba kuchiita kuti kusawirirana kwePRP kurapwa, uye kune misiyano mikuru mukugadzirira kwePRP, mhando yemuenzaniso uye migumisiro yekliniki.Iyo iripo yekutengesa PRP midziyo yakave yakasimbiswa uye yakanyoreswa maererano nezvinodiwa zvemugadziri wevaridzi, iyo inogadzirisa zvakasiyana-siyana pakati peiyo iripo PRP michina.
Nzwisisa platelet dose in vitro uye in vivo
Therapeutic effect yePRP uye mamwe maplatelet anotarisa anobva pakusunungurwa kwezvinhu zvakasiyana-siyana zvinobatanidzwa mukugadzirisa kwenyama uye kuberekwa patsva.Mushure mekushandiswa kweplatelet, maplatelet anoumba platelet thrombus, iyo inozoshanda seyechinguva extracellular matrix kukurudzira kuwedzera kwesero uye kusiyanisa.Naizvozvo, zvine hungwaru kufunga kuti yakakwira platelet dose inotungamira kune yakakwira yemuno kuungana kweplatelet bioactive zvinhu.Zvisinei, kuwirirana pakati peyero uye kuiswa kweplatelet uye kubudiswa kweplatelet bioactive kukura chinhu uye zvinodhaka zvingave zvisingadzoreki, nokuti kune misiyano yakakura mukutanga kweplatelet count pakati pevarwere mumwe nomumwe, uye pane kusiyana pakati pePRP nzira dzekugadzirira.Saizvozvo, akati wandei ekuwedzera kweplatelet zvinhu zvinosanganisirwa mukugadzirisa matishu maitiro aripo mu plasma chikamu chePRP (somuenzaniso, chiropa chekukura chinhu uye insulin-yakafanana nekukura factor 1).Naizvozvo, yakakwirira yeplatelet dose haizokanganisa kugona kugadzirisa kwezvinhu izvi zvinokura.
In vitro PRP tsvagiridzo inofarirwa zvikuru nekuti maparamita akasiyana muzvidzidzo izvi anogona kudzorwa nemazvo uye mhedzisiro inogona kuwanikwa nekukurumidza.Ongororo dzinoverengeka dzakaratidza kuti masero anopindura kuPRP nenzira inoenderana nedosi.Nguyen naPham vakaratidza kuti kuwanda kwakanyanya kweGF kwaisave kukonzeresa maitiro ekusimudzira masero, izvo zvingave zvisingabatsiri.Zvimwe zvidzidzo zvemu vitro zvakaratidza kuti yakakwira PGF kutariswa kunogona kuve nemhedzisiro yakaipa.Chimwe chikonzero chinogona kunge chiri nhamba shoma ye cell membrane receptors.Naizvozvo, kana iyo PGF nhanho yanyanya kukwirira kana ichienzaniswa neanowanikwa receptors, ivo vanozove nemhedzisiro yakaipa pasero basa.
Kukosha kweiyo platelet concentration data in vitro
Kunyangwe in vitro tsvagiridzo ine akawanda mabhenefiti, ine zvakare zvimwe zvayakaipira.In vitro, nekuda kwekuenderera mberi kwekudyidzana pakati pemhando dzakasiyana siyana dzesero mune chero tishu nekuda kwechimiro chetishu uye cellular tishu, zvakaoma kudzokorora in vitro mune maviri-dimensional imwechete tsika nharaunda.Iyo sero density iyo inogona kukanganisa sero chiratidzo nzira inowanzove isingasviki 1% yetishu mamiriro.Maviri-dimensional tsika dish tishu inodzivirira maseru kuti asaonekwe kune extracellular matrix (ECM).Uye zvakare, iyo yakajairika tsika tekinoroji inotungamira mukuunganidzwa kwemarara emasero uye kuenderera kunoshandiswa kwekudya kunovaka muviri.Naizvozvo, in vitro tsika yakasiyana nechero yakatsiga-mamiriro ezvinhu, tishu okisijeni inopa kana kamwe kamwe kuchinjana kwetsika yepakati, uye mhedzisiro inopesana yakabudiswa, ichienzanisa nekiriniki mhedzisiro yePRP neiyo in vitro kudzidza yeakananga maseru, mhando dzematishu uye platelet. concentrations.Graziani nevamwe.Yakawanikwa kuti in vitro, iyo yakanyanya kuitika pakupararira kwe osteoblasts uye fibroblasts yakawanikwa paPRP platelet concentration 2.5 nguva yakakwirira kudarika kukosha kwekutanga.Kusiyana neizvi, data yekiriniki yakapiwa naPark uye vashandi vakaratidza kuti mushure mekuputika kwepelinha, PRP platelet level inoda kuwedzerwa nekanopfuura 5 nguva pane yekutanga kuti iite migumisiro yakanaka.Zvakafanana zvinopesana mhedzisiro zvakataurwa zvakare pakati petendon proliferation data in vitro uye mhedzisiro yekiriniki.
(Zviri muchinyorwa ichi zvakadhindwa patsva, uye isu hatipe chero vimbiso yechokwadi, kuvimbika kana kuzara kwezviri muchinyorwa chino, uye hatina mhosva yemafungiro echinyorwa ichi, ndapota nzwisisa.)
Nguva yekutumira: Mar-01-2023